Contribution of CBX4 to cumulus oophorus cell phenotype in mice and attendant effects in cumulus cell cloned embryos
نویسندگان
چکیده
منابع مشابه
Contribution of CBX4 to cumulus oophorus cell phenotype in mice and attendant effects in cumulus cell cloned embryos.
Cumulus oophorus cells play an essential role in oocyte development. They are also widely employed as donor cells for cloning by somatic cell nuclear transfer. Our previous studies revealed that Cbx4 mRNA was overexpressed in cloned two-cell embryos. These data indicated that CBX4 may regulate normal cumulus cell differentiation and that its overexpression in clones could contribute to aberrant...
متن کاملAbnormal gene expression in cloned mice derived from embryonic stem cell and cumulus cell nuclei.
To assess the extent of abnormal gene expression in clones, we assessed global gene expression by microarray analysis on RNA from the placentas and livers of neonatal cloned mice derived by nuclear transfer (NT) from both cultured embryonic stem cells and freshly isolated cumulus cells. Direct comparison of gene expression profiles of more than 10,000 genes showed that for both donor cell types...
متن کاملProduction of Cloned Mice by Nuclear Transfer of Cumulus Cells
BACKGROUND Over the past several years, mammals have been successfully cloned by either the splitting of an early stage embryo or nuclear transfer of adult somatic cells (NT) into oocytes. Although it has been 15 years since the generation of the first cloned mammals from somatic cells by NT, the success rate for producing live offspring by this technique is low regardless of the cell type and ...
متن کاملEstablishment of a capillary-cumulus model to study the selection of sperm for fertilization by the cumulus oophorus.
BACKGROUND Spermatozoa have to traverse the cumulus oophorus before fertilization in vivo. Evidence suggests that the cumulus oophorus plays an important role in the fertilization process. We describe the establishment of a capillary-cumulus oophorus model with which to study the action of cumulus mass on the function of human spermatozoa. METHODS Human cumulus oophorus was aspirated into a g...
متن کاملproduction of cloned mice by nuclear transfer of cumulus cells
background: over the past several years, mammals have been successfully cloned by either the splitting of an early stage embryo or nuclear transfer of adult somatic cells (nt) into oocytes. although it has been 15 years since the generation of the first cloned mammals from somatic cells by nt, the success rate for producing live offspring by this technique is low regardless of the cell type and...
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ژورنال
عنوان ژورنال: Physiological Genomics
سال: 2014
ISSN: 1094-8341,1531-2267
DOI: 10.1152/physiolgenomics.00071.2013